I. Install ActivePerl (1) download from ftp://ftp.activestate.com/ActivePerl/Windows/ then install II. Install Bioperl (1) go to dos command prompt; run c:\>ppm (2) ppm> rep add Bioperl http://bioperl.org/DIST ppm> rep add Kobes http://theoryx5.uwinnipeg.ca/ppms ppm> rep add Bribes http://www.Bribes.org/perl/ppm ppm> search Bioperl ppm> install Bioperl-1.4 (maybe you like a different version list on screen) (3) ppm> exit (4) for more information, refer to http://www.bioperl.org/Core/Latest/INSTALL.WIN III. Test Bioperl (1) run the following perl program use Bio::Perl; # this script will only work if you have an internet connection on the # computer you're using, the databases you can get sequences from # are 'swiss', 'genbank', 'genpept', 'embl', and 'refseq' $seq_object = get_sequence('swiss',"ROA1_HUMAN"); write_sequence(">roa1.fasta",'fasta',$seq_object); (2)If roa1.fasta can be found at the same direcotry as the perl scirpt, congralutions, you make it!!!!